ÄKTA avant 是一种制备级蛋白纯化层析系统，设计用于快速、安全地开发可扩大性蛋白方法和工艺。ÄKTA avant是用于方法探索和纯化工艺优化的全自动智能液相色谱系统，现有两个型号。ÄKTA avant两款系统具有不同的流速/压力参数，但具有相同的硬件装置。ÄKTA avant 25系统设计流速为25mL/min，用于填料筛选，方法优化和数百毫克级样品分析和制备。ÄKTA avant 150系统设计流速为150mL/min，可用于填料筛选方法优化和工艺放大，以及克级样品的分析和制备。
ÄKTA avant 全自动智能层析系统
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ÄKTA avant 25
| 参数 | ÄKTA avant 25 |
|---|---|
| Application | Fast and secure process development and method optimization |
| Application supported | Affinity chromatography, gel filtration/size exclusion chromatography, ion exchange chromatography, and hydrophobic interaction chromatography |
| Automatic buffer preparation | BufferPro |
| Autosampler injection | No |
| Buffer selection | 18 buffer inlets, option of up to 46 |
| Compliance | The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva and connected only to other CE labeled Cytiva modules or other products as recommended. For regulatory details please see declaration of conformity. |
| Depth | 710 mm |
| Flow cell path length | 2 mm (optional 10 mm) |
| Flow rate | 0.001 mL/min-25 mL/min |
| Height | 660 mm |
| Includes | Accessory box, 3 mixer chambers (0.6, 1.4 and 5 mL), pH electrode, fraction collector cassette tray, 4 deep-well cassettes, 2 cassettes for 50mL tubes |
| Multiple sample injection | Yes, up to 7 samples |
| Multiple wavelength detection | Yes, up to 3 wavelengths |
| Multistep purification | Yes (optional) |
| Operating pressure (sample pump) max. | 10 MPa (100 bar, 1450 psi) |
| Operating pressure max. | 20 MPa (200 bar, 2900 psi) |
| Power consumption | 800 VA |
| Protection class | IP 21, wet side IP 22 |
| Pump type | Piston pump, metering type |
| Relative humidity | 20% to 95% (non condensing) |
| Safety certifications | 2006/42/EC (MD), 2004/108/EC (EMC), 1999/5/EC (R&TTE) |
| Tubing i.d. (flow path) | 0.75 mm |
| UV wavelength | 190–700 nm |
| Viscosity | 0.35–10 cP (system pump), 0.7–10 cP (sample pump) |
| Voltage | 100–120/220–240 VAC |
| Width | 860 mm |
| pH monitoring | 0–14 |
| Model | ÄKTA avant 25 |
ÄKTA avant 150
| 参数 | ÄKTA avant 150 |
|---|---|
| Application | Fast and secure process development and scale-up |
| Application supported | Affinity chromatography, gel filtration/size exclusion chromatography, ion exchange chromatography, and hydrophobic interaction chromatography |
| Automatic buffer preparation | BufferPro |
| Autosampler injection | No |
| Buffer selection | 18 buffer inlets, option of up to 46 |
| Compliance | The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva and connected only to other CE labeled Cytiva modules or other products as recommended. For regulatory details please see declaration of conformity. |
| Depth | 710 mm |
| Flow cell path length | 2 mm (optional 10 mm and 0.5 mm) |
| Flow rate | 0.01 mL/min-150 mL/min |
| Height | 660 mm |
| Includes | Accessory box, 3 mixer chambers (1.4, 5 and 15 mL), pH electrode, fraction collector cassette tray, 2 cassettes for 15 mL tubes, 4 cassettes for 50 mL tubes. |
| Multiple sample injection | Yes, up to 7 samples |
| Multiple wavelength detection | Yes, up to 3 wavelengths |
| Multistep purification | No |
| Operating pressure (sample pump) max. | 5 MPa (50 bar, 725 psi) |
| Operating pressure max. | 5 MPa (50 bar, 725 psi) |
| Power consumption | 800 VA |
| Protection class | IP 20 |
| Pump type | Piston pump, metering type |
| Relative humidity | 20% to 95% (noncondensing) |
| Safety certifications | 2006/42/EC (MD), 2004/108/EC (EMC), 1999/5/EC (R&TTE) |
| Tubing i.d. (flow path) | 0.75 mm |
| UV wavelength | 190–700 nm |
| Viscosity | 0.35 to 5 cP (system pump), 0.7 to 10 cP (sample pump) |
| Voltage | 100–120/220–240 VAC |
| Width | 860 mm |
| pH monitoring | 0–14 |
| Model | ÄKTA avant 150 |
